Search results for "isotopologue profiling"

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Dynamics of Monoterpene Formation in Spike Lavender Plants

2017

The metabolic cross-talk between the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways was analyzed in spike lavender (Lavandula latifolia Med) on the basis of 13CO2-labelling experiments using wildtype and transgenic plants overexpressing the 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), the first and key enzyme of the MVA pathway. The plants were labelled in the presence of 13CO2 in a gas chamber for controlled pulse and chase periods of time. GC/MS and NMR analysis of 1,8-cineole and camphor, the major monoterpenes present in their essential oil, indicated that the C5-precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of both monoterpenes …

0106 biological sciences0301 basic medicineLavenderEndocrinology Diabetes and MetabolismMonoterpeneLavandula latifolialcsh:QR1-502Reductase01 natural sciencesBiochemistryArticlelcsh:Microbiologylaw.inventionessential oils; isotopologue profiling; lamiaceae; <i>Lavandula latifolia</i>; spike lavender; terpenoid biosynthesis; mevalonate; CO<sub>2</sub>terpenoid biosynthesislamiaceae03 medical and health sciencesCamphorchemistry.chemical_compoundmevalonatelawMolecular Biologyessential oilsEssential oilchemistry.chemical_classificationbiologyChemistrybiology.organism_classificationddc:spike lavender030104 developmental biologyEnzymeisotopologue profilingBiochemistryLamiaceaeCO2Lavandula latifolia010606 plant biology & botanyMetabolites
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